, plus the B-10 primer generated polymorphic bands ranging from 900 to 1,450 base

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Only two branches, A and B, had been composed mainly of isolates from Campo GrandeTable 1 Leishmania isolates obtained from stray dogs collected in two endemic places of visceral leishmaniasis in BrazilCode Year Origin1J* 2J* 3J* 4J* 5J* 6J* 7J* 8J* 10J* 11J* 12J* 13J* 14J* 15J* 16J* 17J* 18J* 19J* 20J* 21J* 1CG* 2CG* 3CG 4CG* 5CG 6CG* 7CG 8CG* 9CG* 10CG* 11CG* 12CG* 13CG* 14CG* 15CG* 16CG* 17CG* 18CG* 19CG* 20CG* 21CG* 23CG* 24CG* 25CG*2006 2006 2006 2006 2006 2006 2006 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie Campo Lculated from a child's mapped household to the closest health Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande jir.2011.0094 Campo Grande Campo Grande Campo Grande Campo Grande Campo fpsyg.2015.00360 Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande*Isolates have been also used within the MLMT evaluation. A comparison of your RAPD profiles identified 24 genotypes amongst the 44 isolates (Figure 2). The genetic variance amongst these isolates ranged from 17.39 to 3.44 . Most of the neighbor-joining branches contained a mix of isolates from ?both Jequie and Campo Grande. Only two branches, A and B, were composed mainly of isolates from Campo GrandeTable 1 Leishmania isolates obtained from stray dogs collected in two endemic regions of visceral leishmaniasis in BrazilCode Year Origin1J* 2J* 3J* 4J* 5J* 6J* 7J* 8J* 10J* 11J* 12J* 13J* 14J* 15J* 16J* 17J* 18J* 19J* 20J* 21J* 1CG* 2CG* 3CG 4CG* 5CG 6CG* 7CG 8CG* 9CG* 10CG* 11CG* 12CG* 13CG* 14CG* 15CG* 16CG* 17CG* 18CG* 19CG* 20CG* 21CG* 23CG* 24CG* 25CG*2006 2006 2006 2006 2006 2006 2006 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie ?Jequie Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande jir.2011.0094 Campo Grande Campo Grande Campo Grande Campo Grande Campo fpsyg.2015.00360 Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande Campo Grande*Isolates were also used in the MLMT evaluation. The above isolates had been used for RAPD analysis.?or Jequie, respectively. Bootstrap evaluation showed low values (below 50 ) for most in the branches. Only the branch connecting the outgroup strains (L. amazonensis and L. braziliensis) using the 44 L. infantum isolates presented important bootstrap values (86 ) (Figure 2). In sum, the tree revealed a predominantly mixed pattern of distribution of the isolates in relation to the geographical origin and also didn't present any correlation with all the year of parasite isolation (not shown). Assessment of genetic diversity amongst L. infantum canine isolates in Jequie and Campo Grande making use of the MLMT ?approach.